Researchers have recently reported the growth of multiple cell lines in serum-free media that substitutes nutrients, growth factors, and hormones for serum.

Leydig and Sertoli cells were successfully cultivated in a serum-free medium containing insulin, transferrin, epidermal growth factor, luteinizing hormone or follicle stimulating hormone, somatomedin, and growth hormone, according to Mather and Sato (BBRC, 1985).

 In this article, we will explore DMEM F12k in detail.

Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 is a reliable option for a variety of cell culture applications, as it is well-suited for a wide range of cell types with demonstrated remarkable outcomes with cell lines like human bone marrow SH-SY5Y and mouse muscle myoblast C2C12.

Let’s begin!

What is Dulbecco’s Modified Eagle Medium F12?

• The mixture known as DMEM/F12 medium contains 21 amino acids, 10 vitamins, glucose, iron, and zinc, among other components. DMEM/F12 is a richer and more complex media than DMEM.

• DMEM/F12 medium is a 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture.

• It is a serum-free medium that contains insulin, transferrin, epidermal growth factor, luteinizing hormone or follicle stimulating hormone, somatomedin, and growth hormone first reported by Mather and Sato. In this article, we will explore DMEM F12k in detail.

Benefits of using Dulbecco’s Modified Eagle Medium F12

• Proven Utility: Effectively supports the growth and multiplication of all cell types

• Rich Nutrient Profile: Packed with vitamins and minerals are critical for cell culture

• Equilibrated Mixture: Provides a well-balanced combination of Dulbecco’s Modified Eagle’s Medium and Nutrient Mixture. F-12 Ham

Product Features and Benefits

• Optimized Composition: A balanced combination of essential nutrients, such as amino acids, carbohydrates, lipids, and inorganic salts, are present in Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12. This mix is critical for cell growth and health. Its composition guarantees cells all they need to carry out their regular metabolic functions effectively.

• Dual Mixture: Dulbecco’s Modified Eagle’s Medium and Ham’s Nutrient Mixture F12 are combined 1:1 in this dual mixture. By enhancing the nutritional profile, this mixing produces a flexible medium that effectively supports a diverse range of cell types.

• Guaranteed Quality: Produced by strict quality control guidelines to deliver dependable and consistent outcomes. The liquid formulation makes handling simple and lowers the possibility of contamination when preparing cultures.

This adaptable medium is used in a wide range of biotechnological and scientific applications. It is used for drug screening, assays for cell proliferation, differentiation investigations, and general cell culture. Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 is a vital tool for cell biology research and drug development because it works with a variety of cell types.

To ensure ideal cell growth and viability when using this medium, follow the suggested protocols and guidelines, which include keeping the environment sterile and pH levels at the right values. Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 is a vital tool for sustaining robust cell cultures and advancing the field of cell biology because it has the perfect balance of nutrients.